Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference |
Identifiers | Additional information |
|---|---|---|---|---|
| MUC2KO, C57BL/6 mice (female) |
MUC2KO | Eugene Chang Lab (University of Chicago) provided initial breeding pairs which were provided to them from Leonard H. Augenlicht at the Department of Oncology of Albert Einstein Cancer Center |
Genotyping was performed by Transnetyx Inc.; Western blot was done to confirm lack of MUC2 (See Figure 5E) |
|
| Rag1KO, C57BL/6 mice (male) |
Rag1KO | Provided by Mazmanian Lab at Caltech |
RRID:IMSR_JAX:002216 | Western blot was done to confirm lack of IgA as explained in the text (See Figure 6E) |
| C57BL/6 mice (all male except for WT controls in MUC2KO experiments in Figure 5) |
WT | The Jackson Laboratory |
RRID:IMSR_JAX:000664 | |
| Antibody | MUC2 polyclonal antibody (rabbit host) |
Biomatik | Cat No: CAU27315 |
|
| Antibody | Li-Cor IRDye 800 CW Goat Anti -Rabbit IgG |
Li-Cor | P/N 925–32211; RRID:AB_2651127 |
|
| Antibody | Li-Cor IRDye 800 CW Goat Anti -Mouse IgG |
Li-Cor | P/N 925–32210; RRID:AB_2687825 |
|
| Antibody | Li-Cor IRDye 800 CW Goat Anti -Mouse IgM |
Li-Cor | P/N 925–32280 | |
| Antibody | Goat Anti-Mouse IgA-unlabeled |
Southern Biotech |
Cat No: 1040–01 | |
| Antibody | Li-Cor IRDye 800 CW Donkey Anti-Goat IgG |
Li-Cor | P/N 925–32214; RRID:AB_2687553 |
|
| Chemical compound, drug |
apple pectin | Solgar Inc. | ‘Apple pectin powder’; SOLGB70120 00B |
|
| Chemical compound, drug |
Fibersol-2 | Archer Daniels Midland/ Matsutani LLC |
Product code: 013100, Lot #: CY4P28540 |
|
| Chemical compound, drug |
USP grade sucrose |
Sigma-Aldrich | ||
| Chemical compound, drug |
Protease inhibitor cocktail |
Roche cOmplete, Mini, EDTA-free Protease-Inhibitor cocktail, Roche |
||
| Chemical compound, drug |
PEG 100 kDa | Dow | POLYOX WSR N-10 |
|
| Chemical compound, drug |
PEG 1 MDa | Dow | POLYOX WSR N-12K |
|
| Chemical compound, drug |
PEG 3350 | Bayer | MiraLAX | |
| Chemical compound, drug |
Hanks' Balanced Salt Solution (without calcium, magnesium, phenol red) |
GE Healthcare Life Sciences |
Product code: SH30588.02 |
|
| Software, algorithm |
3D aggregate analysis pipeline |
This paper; source code available through Dryad |
Description in Materials and methods; source code provided on Dryad |
|
| Other | mesh-bottom (or wire-bottom) floors |
Lab Products, Inc. |
P/N: 75016 | |
| Other | 1 μm diameter PEG 5 kDa-coated polysytrene beads |
This paper | Description of synthesis in Materials and methods |
|
| Other | 1 μm diameter PEG 5 kDa-coated polysytrene beads with PEG 1 kDa ‘back-filling’ |
This paper | Description of synthesis in Materials and methods |
|
| Other | standard chow diet |
PicoLab | PicoLab Rodent Diet 20; Product #5053 |
|
| Other | autoclaved chow diet |
PicoLab | Laboratory Autoclavable Rodent Diet 5010 |