Table 2. Estimates of physical parameters of polymers from gel permeation chromatography for liquid fractions from the upper small intestine of MUC2 knockout (MUC2KO) and wild-type (WT) mice.
| Retention volume (mL) | 11 to 16 | 16 to 20 | >20 | ||||
|---|---|---|---|---|---|---|---|
| Mouse type | WT | MUC2KO | WT | MUC2KO | WT | MUC2KO | |
| Mw (kDa) | 3,560 ± 410 | 5,420 ± 620 | 162 ± 20 | 147 ± 17 | 4.05 ± 0.46 | 2.96 ± 0.34 | |
| Mw/Mn | 1.36 | 1.59 | 2.16 | 2.43 | 3.59 | 10.9 | |
| Rh (nm) | 49.1 | 45.5 | 6.31 | 5.95 | 1.18 | 1.02 | |
| Fract. Conc. (mg/mL) | 2.52 ± 0.29 | 1.18 ± 0.13 | 24.6 ± 2.8 | 21.9 ± 2.5 | 88.7 ± 10.1 | 86.0 ± 9.8 | |
We calculated values with both dn/dc = 0.185 (for proteins) and dn/dc = 0.147 (pullulan). When the value varied with dn/dc, it is reported in the table as the mid-range values ± the absolute deviation between the two calculated values. Mw = the weight-average molecular weight; Mw/Mn = the dispersity; Rh = hydrodynamic radius; Fract. Conc. = Concentration of a given molecular weight fraction.