Table 3. Estimates of physical parameters of polymers from gel permeation chromatography for liquid fractions from the lower small intestine of MUC2 knockout (MUC2KO) and wild-type (WT) mice.
| Retention volume (mL) | 11 to 16 | 16 to 20 | >20 | |||
|---|---|---|---|---|---|---|
| Mouse type | WT | MUC2KO | WT | MUC2KO | WT | MUC2KO |
| Mw (kDa) | 4,730 ± 540 | 5,180 ± 590 | 219 ± 25 | 155 ± 18 | 13.7 ± 1.6 | 5.93 ± 0.68 |
| Mw/Mn | 1.24 | 1.80 | 1.91 | 1.84 | 1.88 | 2.03 |
| Rh (nm) | 57.0 | 49.2 | 8.45 | 7.58 | 1.89 | 1.35 |
| Fract. Conc. (mg/mL) | 3.42 ± 0.39 | 2.36 ± 0.27 | 23.0 ± 2.6 | 22.8 ± 2.6 | 54.8 ± 6.3 | 63.3 ± 7.2 |
We calculated values with both dn/dc = 0.185 (for proteins) and dn/dc = 0.147 (pullulan). When the value varied with dn/dc, it is reported in the table as the mid-range values ± the absolute deviation between the two calculated values. Mw = the weight-average molecular weight; Mw/Mn = the dispersity; Rh = hydrodynamic radius; Fract. Conc. = Concentration of a given molecular weight fraction.