Table 4. Estimates of physical parameters of polymers from gel permeation chromatography for liquid fractions from the upper small intestine of immunoglobulin-deficient (Rag1KO) and wild-type (WT) mice.
| Retention volume (mL) | 11 to 16 | 16 to 20 | >20 | |||
|---|---|---|---|---|---|---|
| Mouse type | WT | Rag1KO | WT | Rag1KO | WT | Rag1KO |
| Mw (kDa) | 1,480 ± 170 | 2,140 ± 250 | 108 ± 12 | 74.2 ± 8.5 | 2.84 ± 0.32 | 1.91 ± 0.22 |
| Mw/Mn | 1.09 | 1.14 | 2.62 | 2.42 | 1.59 | 1.54 |
| Rh (nm) | 31.8 | 39.8 | 4.77 | 2.51 | 1.078 | 0.936 |
| Fract. Conc. (mg/mL) | 1.07 ± 0.12 | 1.13 ± 0.13 | 14.3 ± 1.6 | 13.9 ± 1.6 | 66.1 ± 7.6 | 70.5 ± 8.1 |
We calculated values with both dn/dc = 0.185 (for proteins) and dn/dc = 0.147 (pullulan). When the value varied with dn/dc, it is reported in the table as the mid-range value ± the absolute deviation between the two calculated values. Mw = the weight-average molecular weight; Mw/Mn = the dispersity; Rh = hydrodynamic radius; Fract. Conc. = Concentration of a given molecular weight fraction.