Table 5. Estimates of physical parameters of polymers from gel permeation chromatography for liquid fractions from the lower small intestine of immunoglobulin-deficient (Rag1KO) and wild-type (WT) mice.
| Retention volume (mL) | 11 to 16 | 16 to 20 | >20 | |||
|---|---|---|---|---|---|---|
| Mouse type | WT | Rag1KO | WT | Rag1KO | WT | Rag1KO |
| Mw (kDa) | 1,080 ± 120 | 2,490 ± 290 | 66.9 ± 7.7 | 91.6 ± 10.5 | 3.64 ± 0.42 | 3.72 ± 0.43 |
| Mw/Mn | 1.18 | 1.05 | 1.71 | 1.98 | 2.09 | 1.98 |
| Rh (nm) | 34.6 | 47.1 | 4.67 | 4.85 | 1.116 | 1.09 |
| Fract. Conc. (mg/mL) | 1.52 ± 0.17 | 1.89 ± 0.22 | 15.8 ± 1.8 | 14.1 ± 1.6 | 49.5 ± 5.7 | 55.1 ± 6.3 |
We calculated values with both dn/dc = 0.185 (for proteins) and dn/dc = 0.147 (pullulan). When the value varied with dn/dc, it is reported in the table as the mid-range values ± the absolute deviation between the two calculated values. Mw = the weight-average molecular weight; Mw/Mn = the dispersity; Rh = hydrodynamic radius; Fract. Conc. = Concentration of a given molecular weight fraction.