Table 7. Estimates of physical parameters of polymers from gel permeation chromatography for liquid fractions from upper small intestine of pectin and Fibersol-2 fed mice.
| Retention volume (mL) | 11 to 16 | 16 to 20 | >20 | |||
|---|---|---|---|---|---|---|
| Mouse type | Pectin | Fibersol-2 | Pectin | Fibersol-2 | Pectin | Fibersol-2 |
| Mw (kDa) | 267 ± 31 | 686 ± 79 | 40.0 ± 4.5 | 35.3 ± 4.0 | 1.39 ± 0.16 | 1.67 ± 0.19 |
| Mw/Mn | 1.50 | 1.08 | 2.15 | 2.64 | 2.45 | 1.48 |
| Rh (nm) | 31.8 | N/C** | 5.52 | 2.88 | 0.819 | N/C** |
| Fract. Conc. (mg/mL) | 1.62 ± 0.19 | 0.516 ± 0.059 | 9.00 ± 1.03 | 23.3 ± 2.7 | 53.7 ± 6.1 | 77.0 ± 8.8 |
We calculated values with both dn/dc = 0.185 (for proteins) and dn/dc = 0.147 (pullulan). When the value varied with dn/dc, it is reported in the table as the mid-range values ± the absolute deviation between the two calculated values. Mw = the weight-average molecular weight; Mw/Mn = the dispersity; Rh= hydrodynamic radius; Fract. Conc. = Concentration of a given molecular weight fraction. N/C** denotes values for which the concentration was too low to calculate.