Table 8. Estimates of physical parameters of polymers from gel permeation chromatography for liquid fractions from lower small intestine of pectin and Fibersol-2-fed mice.
| Retention volume (mL) | 11 to 16 | 16 to 20 | >20 | |||
|---|---|---|---|---|---|---|
| Mouse type | Pectin | Fibersol-2 | Pectin | Fibersol-2 | Pectin | Fibersol-2 |
| Mw (kDa) | 282 ± 32 | 1680 ± 190 | 30.2 ± 3.5 | 18.8 ± 2.2 | 1.12 ± 0.13 | 2.32 ± 0.27 |
| Mw/Mn | 7.37 | 1.64 | 1.70 | 2.78 | 2.89 | 1.14 |
| Rh (nm) | 29.0 | 26.4 | 5.28 | 2.16 | 0.724 | 1.06 |
| Fract. Conc. (mg/mL) | 2.48 ± 0.28 | 0.839 ± 0.096 | 9.43 ± 1.1 | 53.6 ± 6.1 | 42.7 ± 4.9 | 88.3 ± 10.1 |
We calculated values with both dn/dc = 0.185 (for proteins) and dn/dc = 0.147 (pullulan). When the value varied with dn/dc, it is reported in the table as the mid-range values ± the absolute deviation between the two calculated values. Mw = the weight-average molecular weight; Mw/Mn = the dispersity; Rh = hydrodynamic radius; Fract. Conc. = concentration of a given molecular weight fraction.