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. 2019 Jan 22;8:e39982. doi: 10.7554/eLife.39982

Figure 8. Increased frequency of Th17 cells in the gut of T300A ASF mice associated with B. ovatus 8483.

LP flow cytometry of T cell and myeloid cell populations from ASF mice (WT n = 6 and T300A n = 6) or ASF mice associated with ~109 CFU of B. ovatus 8483 for 3 weeks (WT n = 6 and T300A n = 6). T cells gated as the frequency of RORγt+ (Th17), T-bet+ (Th1), Foxp3+ (Treg) or GATA-3+ (Th2) cells out of CD3+CD4+ cells and myeloid cell gated as the frequency of CD11c+MHCII+ cells out of CD45+ cells (conventional DCs), CD11b-CD103+ cells out of CD11c+MHCII+ cells (tolerogentic DCs), CD11b+GR-1int cells out of CD45+ cells (neutrophils), and CD11b+GR-1hi cells out of CD45+ cells (inflammatory monocytes). CD3+CD4+ cell and myeloid cell populations were gated on single, live, CD45+ lymphocytes. (a) ASF colonic LP T cells (top) and myeloid cells (bottom) (b) ASF +B. ovatus 8483 colonic LP T cells (top) and myeloid cells (bottom). (c) ASF ileal LP T cells (top) and myeloid cells (bottom) (d) ASF +B. ovatus 8483 ileal LP T cells (top) and myeloid cells (bottom). Data are plotted as the mean ± SEM. ns = not significant. Mann-Whitney U test.

Figure 8—source data 1. Source data for Figure 8.
elife-39982-fig8-data1.xlsx (40.1KB, xlsx)
DOI: 10.7554/eLife.39982.021

Figure 8.

Figure 8—figure supplement 1. Immune infiltration in ASF mice associated with B. ovatus occurs in the absence of disease.

Figure 8—figure supplement 1.

WT and T300A ASF mice were associated ~109 CFU of B. ovatus 8483 for 3 weeks (WT n = 6, T300A n = 6). Histologic colitis/ileitis scores were calculated by grading monocyte infiltration, epithelial hyperplasia, epithelial injury and polymorphonuclear (PMN) cell infiltration, all from 0 to 3 for a maximum total score of 12. Histologic colitis/ileitis scores from (a) the colon and (b) the ileum of ASF mice or ASF mice associated with B. ovatus 8483. Data are plotted as mean ± SEM.
Figure 8—figure supplement 2. No differences in colonization levels of B. ovatus 8483 in GF or ASF mice.

Figure 8—figure supplement 2.

GF mice (WT n = 6, T300A n = 6) or ASF mice (n = 6, T300A n = 6) were associated with ~109 CFU of B. ovatus 8483 for three wks. Fecal samples were taken at the three wk timepoint and CFU/g stool was calculated. ns = not significant. Two-way ANOVA with Tukey’s post-hoc test.