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Use of lactone protection as a strategy to provide regioselectivity for chemoenzymatic synthesis of ganglioside disialyl tetrasaccharide. In the absence of lactone protection, mixture of disialyl tetrasaccharides and trisialyl pentasaccharide were formed in Photobacterium damselae α2–6-sialyltransferase (Pd2,6ST)-catalyzed reaction.
