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. Author manuscript; available in PMC: 2020 Feb 1.
Published in final edited form as: Kidney Int. 2018 Dec 3;95(2):310–320. doi: 10.1016/j.kint.2018.08.040

Figure 3. Removal of KLN results in protection from kidney injury in NTN.

Figure 3.

(A) Representative figure of H&E stained kidney at NTN Day 7 shows glomerulus with crescent in control mouse that underwent sham surgery and normal appearance of glomerulus in kidney of mouse following KLN removal. Scale bar 50 µm. (B) Percentage of crescentic glomeruli in kidneys at Day 7 decreases following KLN removal (Control: n=5, mean 44.96%±10.44, range 17.85714–80.76923%, median 42.85714%; KLN removed: n=4, 12.5%±4.279, 0– 19.04762%, median 15.476205%). (C) Infiltration of CD3+ T cells (red, top row) and F4/80+ macrophages (red, bottom row) decreases in kidney following KLN removal Glomerulus marker nephrin (green), nuclear marker DAPI (blue). Scale bar 50 µm. (D) CD3+ and F4/80+ cell counts decrease following KLN removal (CD3+ T cells: Control: n=5, 53.4±7.966, 23–69, median 60; KLN removed: n=4, 11.5±1.19, 9–14, median 11.5) (F4/80+ macrophages: Control: n=5, 149.8 ±9.82, 120–173, median 160; KLN removed: n=4, 73±7.405, 57–90, median 72.5). (E) BUN at Days 2 and 7 is reduced in mice following KLN removal (Day 2: Control: n=5, 88.91±10.68, 48.57907–107, median 101; KLN removed: n=4, 35.5±8.649, 16.137360–58.14828, median 33.853445) (Day 7: Control: n=5, 37±2.627, 30–43,median 36; KLN removed: n=4, 27.43±2.028, 22.54018–31.32203, median 27.93848) (p=0.0635 for Day 7). (F) Glomerulus with crescent in control mouse that underwent sham surgery and normal appearance of glomerulus in kidney of mouse following KLN removal in the absence of pre-immunization. (G) In the non-immunized NTN model, the percentage of crescentic glomeruli in kidneys at Week 3 decreases following KLN removal (Control: n=4, 28.16%±4.9444, 20–42.30769%, median 25.174825; KLN removed: n=4, 11.77%±3.06, 4.166667 17.391300%, median 6.845238%). (H) Infiltration of F4/80+ macrophages (red) decreases in kidney following KLN removal in the absence of pre-immunization at Day 3. Glomerulus marker nephrin (green), nuclear marker DAPI (blue). Scale bar 50 µm. (I) F4/80+ macrophage cell counts decrease following KLN removal in the absence of pre-immunization at Day 3 (Control: n=4, 101.3±13.35, 81–138, median 121; KLN removed: n=4, 43.75±4.11, 39–56, median 47.5). *p<0.05. KLN: kidney lymph node, NTN: nephrotoxic serum nephritis, H&E: hematoxylin and eosin, BUN: blood urea nitrogen. Data in graphs are presented as means (horizontal bar) ± SEM (vertical bars).