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. Author manuscript; available in PMC: 2020 Feb 1.
Published in final edited form as: Curr Protoc Protein Sci. 2018 Oct 12;95(1):e76. doi: 10.1002/cpps.76

Figure 2. Western blotting detection of protein S-sulfenylation in RKO cells.

Figure 2.

Intact RKO cells were labeled with the BTD probe for the indicated concentration (A) and time (B). BTD-labeled S-sulfenylated proteins can be conjugated to azide-biotin via CuAAC and detected by streptavidin-HRP immunoblot. Equal protein loading is demonstrated by Coomassie G-250 dye staining of the SDS-PAGE gel.