. Author manuscript; available in PMC: 2020 Feb 1.

Published in final edited form as: Curr Protoc Protein Sci. 2018 Oct 12;95(1):e76. doi: 10.1002/cpps.76

Table 1.

Major parameters used for S-sulfenylated peptide identifcation and protein assembly

Parameters	Settings
Database	Uniprot Homo Sapiens canonical database
Enzyme	Trypsin KR_C (Full-specific)
Maximum number of missed cleavages	3
Precursor tolerance	20 ppm
Fragment tolerance	20 ppm
Dynamic modification	Methionine oxidation (+15.9949 Da) Carbamidomethylation (+57.0214 Da) BTD (C₁₃ ¹²C₆ H₂₂ N₄ O₅ S, +418.1311 Da)
Quantitation type	Labeling-SILAC etc
Multiplicity	2
FDR	Less than 1 % at peptides level
Peptide mass	[600, 10000]
Peptide length	[6,100]
Number of peptides per protein	At least 1
Protein FDR	1%
Open Search	False