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. 2018 Nov 22;116(1):142–150. doi: 10.1016/j.bpj.2018.11.022

Figure 1.

Figure 1

(A) Images of Lifeact-mCherry (LA-mCherry, left) and EGFP-actin (middle) in live Xenopus XTC cells. After fixation, F-actin was stained with Alexa 647 phalloidin (A647-phalloidin, right). Representative data are shown (n = 6 cells). (B) Merged images of (A) showing rear-biased distribution of LA-mCherry in lamellipodia are given. (C) The average fluorescence intensity of the images in (A) along the yellow lines in the inset is shown. (D and E) Similar distribution of Atto-550 Lifeact (Atto-550-LA) and rhodamine-phalloidin (Rh-phalloidin) in fixed XTC cells is shown. Staining with Rh-phalloidin was carried out after washing out Atto-550 Lifeact, which is an exchangeable probe (6). Representative data are shown (n = 3 cells, see Fig. S1).