Figure 2.

(A) Fluorescent speckle images of Alexa 546 phalloidin (A546-phalloidin, left) and CF680R-actin (middle) in a live fish keratocyte. The probes were delivered into keratocytes by electroporation (15). F-actin was stained with Oregon Green phalloidin (OG-phalloidin) after fixation (right). (B) The average fluorescence intensity of the images in (A) along the white lines in the inset is shown. Representative data are shown (n = 4 cells). The region where the lamellipodium detached from the glass was excluded from analysis (the blue arrow in (A)).