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. 2018 Nov 22;116(1):142–150. doi: 10.1016/j.bpj.2018.11.022

Figure 6.

Figure 6

(A, C, E, and G) Images of Lifeact-EGFP (LA-EGFP) and Alexa 546 phalloidin (A546-phalloidin) in four live Xenopus XTC cells with the retrograde flow speeds indicated. The distributions of LA-EGFP and A546-phalloidin are compared to the F-actin distribution visualized by miRFP703-actin in live cells (A, E, and G) or staining with A546-phalloidin after fixation (C). (B, D, F, and H) The average fluorescence intensity of the images in (A, C, and E) or (G) along the yellow lines in the inset is shown. Representative data are shown (n = 11 cells). Bars, 5 μm.