Fig. 3. PTEN a target of miR-182-5p. (A) Putative binding sites of miR-182-5p and PTEN was predicted by miRTarBase and miRanda. (B–E) The luciferase activity was investigated in MDA-MB-231 and MCF-7 cells co-transfected with WT or MUT luciferase vectors and miR-182-5p, anti-miR-182-5p or their negative control. (F) The abundance of enrichment of PTEN was detected in MDA-MB-231 and MCF-7 cells after Ago1 RNA immunoprecipitation (RIP). (G and H) The expression of PTEN was measured in breast cancer (BC) tissues and cells at the mRNA and protein level, respectively. (I) The effect of miR-182-5p on PTEN protein expression was examined in MDA-MB-231 and MCF-7 cells. ^*p<0.05.