Fig. 3. miR-206 reduces PAX3 and MET expression, as well as Akt1 and Erk1/2 activation. (A–C) RT-qPCR evaluating miR-206, PAX3 mRNA and MET mRNA in OS cells after indicated lentiviral transduction. (D–H) Western blot detecting the protein expression levels of PAX3 and MET, as well as Akt1 Ser473 and Erk1/2 total threonine phosphorylation, in OS cells after indicated transduction. NT was used as control for significance test. OS, osteosarcoma; NT, non-transduced group.