Fig. 4. miR-206 reduces OS cell proliferation while promoting apoptosis in vitro. miR-206 overexpression or knockdown in OS cells in vitro was achieved by lentiviral transduction. (A) CCK-8 cell viability assay evaluating overall cell growth in vitro. (B) Western blot evaluating the protein expression levels of the cell proliferation marker PCNA. (C) Flow cytometry detection of cell apoptosis. (D) Western blot evaluating the activation of pro-apoptotic protein Caspase-3. (E) Representative results of Western blots. NT was used as a control for significance test. ^*p<0.01, ^†p<0.001. OS, osteosarcoma; NT, non-transduced group.