Fig. 7. miR-206 reduces PAX3 and MET gene expression and IP3K-AKT, MAPK-ERK signaling in OS cells. OS cells were treated as indicated in Fig. 6 before Western blot assay (A), Western blot detecting the protein expression levels of Pax3 or c-Met in OS cells after different treatment. (B and C) Western blot detecting Akt1 Ser473 or Erk1/2 total threonine phosphorylation in OS cells after different treatments. (D) Representative Western blot results. WT OS cells were used as a control for significance test.. OS, osteosarcoma; WT, wild-type.
