Figure 3.

(A) A plot of the calculated HDX propensity for the 170s_CT loop (residues 201–223) for WT thrombin (black) as compared to the W215A mutant thrombin (blue). The cutoff line at 6% is shown. (B) A table of the HDXMS results showing the difference in exchange for peptides covering the 170s_CT loop at 0.5 and 5 min. (C) An overlay of the structure of WT thrombin (wheat) with a representative structure of the excited state (blue) in which the 170s_CT loop helix has rearranged. Hydrogen bonds (H-bonds) are shown as dashed lines, and the rearranged region is shown in expanded view. To see this figure in color, go online.