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. 2019 Jan 22;12:2. doi: 10.1186/s12284-018-0260-z

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© The Author(s). 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

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Fig. 4 — Interactions between indicated proteins. a, Interactions between indicated proteins were investigated by Y2H. Plus sign, positive control; minus sign, negative control; QD + AbA, quadruple-dropout medium lacking Ade, His, Leu and Trp containing 200 ng/mL AbA; DD, double-dropout medium lacking Leu and Trp. b, c, d, and e, pull-down results of four pairs of proteins: MKKK62-MKK3, MKKK62-MKK10–2, MKK3-MAPK7, and MKK3-MAPK14, respectively. Binding of GST- and His-fusion proteins was tested by immunoblotting using anti-His after pull-down with GST-fusion protein. GST was used as negative control. Expression of fusion proteins was detected using antibody to the tag. “Input” indicated the detection result of the fusion proteins with anti-His or anti-GST antibody; “Pull-down” indicated the detection result of His-fusion protein binding to GST-fusion protein with anti-His antibody. Arrow indicates the band of the target protein