Fig. 2.

The comparison of GNPs/Ab conjugates prepared at different dates using M180 membrane. Test line: 0.3 μg per strip Salmonella Ab. Control line: 0.6 μg per strip anti goat IgG. The conjugates were prepared by 2 (a), 6 (b) and 8 (c) months old GNPs. Strips A5, C19 and C21 were prepared by naked 1 GNPs coming from Table 1. Sample preparation was performed by mixing 100 μL of overnight culture and 100 μL of PBS (a, b) and the sample was loaded by adding 33 and 25 μL of each overnight growth bacteria into 100 μL PBS as total volume separately for the mixture containing three and four different bacteria samples, respectively (c). Bacteria alone was loaded as 100 μL from overnight culture