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. 2018 Oct 31;266(1):92–101. doi: 10.1007/s00415-018-9084-4

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© The Author(s) 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

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Fig. 4 — Minigene analysis. a RT-PCR analysis of COS1 cells transfected by systems reporting: 1–3. WT minigenes, 4–6. mutated minigenes; 7. Amplified fragments of non-transfected COS-1; M. Molecular weight marker. 234 bp. Amplified fragments containing GBA exon 4; 87 bp. amplified fragments without GBA exon 4. Both fragments (243 bp and 87 bp) were sequenced on both strand. b Normalized Volume quantification obtained with ChemiDocMP imager (Bio-Rad). 1–3 wt minigenes; 4–6. mutated minigenes; WT wt GBA mRNA; MUT Mutated GBA mRNA. Standard deviation is provided