Fig. 4.

a, b Impact of liver enzyme homogenate on the DNA-damaging activity of CBD and CBDV in TR146 cells. The cells were treated with 2.0 µM of the cannabinoids and in parallel with liver enzyme homogenate (for details see “Materials and methods”). Bars indicate means ± SD of results obtained with two parallel cultures per experiment (from each culture two slides were made and 50 cells were evaluated per slide). Stars indicate statistical significance (p ≤ 0.05, Two-tailed paired t test). All statistical calculations are based on comparisons between results which were obtained with cells which had been treated with the test compounds and results which were obtained with corresponding solvent controls