Table 1.
Structure formation frequency and length distribution analysis of indicated yeast strains grown under indicated culture conditions.
| Yeast strain | Clone # | % cells with Asn1p-GFP structures (average ± SEM) | ||
|---|---|---|---|---|
| Log-phase | Day 1 | Day 5 | ||
| ASN1::GFP (from the yeast GFP collection) | — | 0.0 ± 0.00 | 26.3 ± 2.64 | 96.4 ± 0.71 |
| ASN1(WT)::GFP asn2Δ | 1 | 0.0 ± 0.00 | 25.1 ± 0.75 | 96.6 ± 1.22 |
| 2 | 0.0 ± 0.00 | 23.3 ± 2.41 | 97.0 ± 0.29 | |
| asn1(C1A)::GFP asn2Δ | 1 | 0.0 ± 0.00 | 15.6 ± 0.55 | 91.2 ± 4.32 |
| 2 | 0.0 ± 0.00 | 16.9 ± 1.68 | 90.1 ± 5.35 | |
| asn1(R344A)::GFP asn2Δ | 1 | 0.0 ± 0.00 | 1.2 ± 0.30 | 2.3 ± 0.43 |
| 2 | 0.0 ± 0.00 | 1.4 ± 0.45 | 4.7 ± 0.49 | |
| asn1(C1A-R344A)::GFP asn2Δ | 1 | 0.0 ± 0.00 | 1.0 ± 0.39 | 2.9 ± 0.53 |
| 2 | 0.0 ± 0.00 | 0.9 ± 0.23 | 3.4 ± 0.73 | |
| P-value (C1A vs. WT) | — | 0.1211ns (two-tailed) | 0.0810ns (two-tailed) | |
| P-value (R344A vs. WT) | — | 0.0279* (two-tailed) | 0.0066** (two-tailed) | |
| P-value (C1A-R344A vs. WT) | — | 0.0237* (two-tailed) | <0.0001**** (two-tailed) | |
| Yeast Strain | Length of asparagine synthetase structure at day 5 (μm) | |||
| Max | Min | Average | ||
| ASN1::GFP (from the yeast GFP collection) | 3.876 | 0.108 | 0.787 | |
| ASN2::GFP (from the yeast GFP collection) | 2.682 | 0.108 | 0.734 | |
| ASN1(WT)::GFP asn2Δ | 3.086 | 0.108 | 0.864 | |
| asn1(C1A)::GFP asn2Δ | 3.820 | 0.108 | 1.041 | |
Note: 300 structures were randomly captured and analyzed for each strain. “ns” indicates not significant (p>0.05), whereas the asterisks indicate significant difference (p<0.05) between WT and mutants.