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. 2019 Jan 22;9:264. doi: 10.1038/s41598-018-36443-8

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

mmu_circRNA_005186 acted as a miRNA Sponge for miR-124-3p and regulated the expression of Epha2. (a) Predicted binding site of miR-124-3p in mmu_circRNA_005186 and a diagram for construction of mutated reporter plasmid (mmu_circRNA_005186-Mut). (b) Dual-luciferase reporter assay showed that miR-124-3p mimics significantly repressed the luciferase activity of mmu_circRNA_005186-WT but not the mutant one in 293T cells. (c) Predicted binding site of miR-124-3p in Epha2 3′-UTR. And a diagram for construction of mutated reporter plasmid (Epha2 3′-UTR-Mut). (d) Dual-luciferase reporter assay showed that miR-124-3p mimics significantly repressed the luciferase activity of Epha2 3′-UTR-Mut but not the mutant one in 293T cells. (e) After transfection with si-005186, the expression level of mmu_circRNA_005186 was significantly inhibited in RAW264.7 cells. (f) The expression level of miR-124-3p was significantly upregulated after mmu_circRNA_005186 silencing in RAW264.7 cells. (g) The expression level of Epha2 was significantly inhibited after mmu_circRNA_005186 silencing in RAW264.7 cells. *p < 0.05, **p < 0.01.