Table 1.

Diversity statistics summarised by season (warm – summer, autumn; cool – winter, spring) and region (Firth of Thames, Inner Gulf and Outer Gulf) for zooplankton samples.

Plankton	COI						18S
	Total	Warm	Cool	Firth	Inner	Outer	Total	Warm	Cool	Firth	Inner	Outer
n	24 (23)	11 (10)	13 (13)	3 (3)	10 (10)	11 (10)	24 (24)	11 (11)	13 (13)	3 (3)	10 (10)	11 (11)
Reads	153387	74691	78316	31430	55661	65916	112207	60416	51791	14164	39808	58235
OTUN	664	373	590	165	414	535	156	125	97	57	82	128
OTUR	527	279	452	109	317	412	139	114	85	50	74	113
β-Sh ± sd	4.97 ± 0.03	2.86 ± 0.02	3.76 ± 0.03	2.31 ± 0.02	3.59 ± 0.03	3.59 ± 0.03	3.72 ± 0.02	3.16 ± 0.02	2.71 ± 0.01	1.41 ± 0.01	3.05 ± 0.02	2.93 ± 0.01
γ-Sh ± sd	122.95 ± 1.26	62.71 ± 0.80	102.13 ± 1.11	37.22 ± 0.93	70.28 ± 1.00	127.63 ± 1.70	10.65 ± 0.06	10.58 ± 0.09	6.79 ± 0.06	4.69 ± 0.02	9.32 ± 0.07	7.57 ± 0.08
Scat	COI						18S
	Total	Warm	Cool	Firth	Inner	Outer	Total	Warm	Cool	Firth	Inner	Outer
n	16 (15)	4 (4)	12 (11)	0	7	7	15 (13)	5 (5)	10 (8)	0	10 (8)	3 (3)
Reads	230677	117117	112885	—	60556	155840	649334	128832	519762	—	462701	43517
OTUN	810	426	702	—	524	557	228	138	216	—	212	134
OTUR	558	248	499	—	377	348	142	86	129	—	126	75
β-Sh ± sd	6.13 ± 0.05	3.99 ± 0.01	5.12 ± 0.04	—	4.13 ± 0.04	3.69 ± 0.03	3.70 ± 0.02	2.95 ± 0.02	2.91 ± 0.02	—	3.12 ± 0.02	1.89 ± 0.01
γ-Sh ± sd	185.25 ± 1.63	93.73 ± 1.54	169.52 ± 1.91	—	123.91 ± 1.63	15.47 ± 1.59	16.72 ± 0.14	12.11 ± 0.16	14.00 ± 0.16	—	15.74 ± 0.17	8.10 ± 0.15

Statistics are shown by DNA barcode, sample type and include: n; sample size, (n); sample size used to calculate rarefaction-based statistics, Reads; total number of reads, OTU_N; total number of OTUs, OTU_R; rarefied number of OTUs, β; Shannon’s β (β – Sh) and γ (γ – Sh) diversity index, with estimated standard deviations (±sd). Rarefied number of OTUs, β and γ diversity statistics were estimated using 952 reads for COI and 1956 reads for 18S.