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. 2019 Jan 22;9:281. doi: 10.1038/s41598-018-36478-x

Table 3.

Significance of tests to detect differences in zooplankton community and whale scat samples using different underlying distance matrices.

Zooplankton Season Region
ANOVA PERMANOVA ANOVA PERMANOVA
D.F. F-value p-value Bray Jaccard Uni-U Uni-W D.F. F-value p-value Bray Jaccard Uni-U Uni-W
COI 1, 21 5.249 0.042 <0.001 0.001 <0.001 0.025 2, 20 3.716 0.039 0.004 0.003 0.003 0.270
18S 1, 22 0.353 0.559 0.032 0.047 0.032 0.005 2, 21 0.813 0.457 0.005 0.006 0.014 0.008
Whale scat
COI 1, 13 0.415 0.484 0.716 0.716 0.443 0.519 1, 12 0.003 0.776 0.114 0.086 0.041 0.550
18S 1, 11 0.607 0.452 0.331 0.336 0.516 0.152 1, 9 0.037 0.851 0.229 0.322 0.476 0.651

ANOVA: D.F. reflects the degrees of freedom (between and within group), F-value and p-value of the ANOVA of the multivariate homogeneity of groups’ dispersions to test for significant differences in β diversity between season (cool or warm) and regions (Firth, Inner and Outer Gulf for zooplankton; Inner and Outer Gulf for whale scat and matched water control). PERMANOVA reflects the significance of multi-factorial (Season and Region) permutational multivariate analysis of variance, using different underlying data distance matrices reflecting OTU occurrence (Jaccard distance on presence/absence transformed data (Jaccard) and unweighted (Uni-U) UniFrac distance matrices) and relative read abundance (Bray-Curtis distance on fourth root transformed data (Bray); and weighted (Uni-W) UniFrac distance matrices).