Fig. 6. Paclitaxel-independent subcellular relocalization of endogenous septin filaments to microtubules occurs following the combined overexpression of polyglutamylases (TTLL5,11) and septins (2,6,7,9_i1) in diverse cell lines.

a Immunofluorescent images of endogenous SEPT2 (green) and actin (phalloidin; red) in cells. The overlay images show that septin fibers colocalize with actin stress fibers in each cell line. b Immunofluorescent images show endogenous SEPT2 (green), overexpressed SEPT9_i1 and alpha-tubulin (red). Septin filaments relocalize to MTs when polyglutamylases and septins (including SEPT9_i1) are overexpressed in CHO, RPE-1, and HeLa cells and only partially in HuH7 cell line. The images shown are representative of at least three independent experiments. Scale bars = 10 µm