Fig. 3. Depletion of eIF5B enhances TRAIL-induced apoptosis without affecting cell cycle progression.

U343 cells were treated as in Fig. 1 before analyzing cellular DNA content by propidium iodide staining and flow cytometry. a Representative images showing the number of cells in G₀/G₁, S, or G₂/M phases, as well as sub-G₀ cells. b Percentage of sub-G₀ cells (top left), cells in G₀/G₁ (top right), S-phase (bottom left), and G₂/M (bottom right). siC, non-specific siRNA; si5B, eIF5B-specific siRNA. Data are expressed as mean ± SEM for four independent biological replicates. *, p < 0.05; **, p < 0.01