Fig. 7. eIF5B promotes translation of XIAP, Bcl-xL, cIAP1, c-FLIPS, Nrf2, and p21 in U343.
a Total RNA was isolated from control or eIF5B-depleted cells and subjected to RT-qPCR analysis to quantitate steady-state levels of cIAP1, c-FLIPL, c-FLIPS, Bcl-xL, XIAP, Nrf2, and p21 mRNAs, all normalized to β-actin mRNA. b Polysome profiling analysis of the aforementioned mRNAs. The proportion of each mRNA (relative to β-actin) for each fraction is shown in Figure S8. Fractions 1–3, representing monosomes, were pooled, as were fractions 4–10, representing polysomes. Data are expressed as mean ± SEM for three independent biological replicates. *, p < 0.05; **, p < 0.01; ***, p < 0.001