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. 2019 Jan 22;9:300. doi: 10.1038/s41598-018-36391-3

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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In vitro binding analysis. (A) Radar plot of the binding profile for displacement of the fluorescent ligand 1-NPN from MUP mutants. The equilibrium association constants, K_a, relative to the binding of each ligand to each mutant MUP are plotted on a logarithmic scale. (B) The equilibrium dissociation constants, K_d, are plotted as a heatmap, displaying the variations across mutants, and between different ligands binding to the same mutant. The change of K_d values are represented from light green to light red and the numbers refer to the range of dissociation constants (µM).