Fig. 1.

sAAV-Tmc1 restores sensory transduction in Tmc1^∆/∆;Tmc2^∆/∆ IHCs and OHCs. a Confocal images of two apical sections excised from P7 Tmc1^∆/∆;Tmc2^∆/∆ mouse cochleas after injection of sAAV-Tmc1 through the RWM at P1. The tissue was cultured for 7 days and perfused with 5 µM FM1-43 for 10 s followed by 3 full bath exchanges to washout excess dye. The tissue was then mounted and imaged for FM1-43 uptake (green) in IHCs and OHCs. Scale bar = 25 µm. b Representative families of sensory transduction currents evoked by mechanical displacement of hair bundles (protocol shown below) recorded from P7 apical OHCs of Tmc1^∆/∆;Tmc2^∆/∆ mice injected with sAAV-Tmc1 that were FM1-43-negative (left) or FM1-43-positive (second from left). FM1-43-positive currents were also recorded from P9 IHCs (second from right) and P30 IHCs (right) of Tmc1^∆/∆;Tmc2^∆/∆ mice injected with sAAV-Tmc1. c Stimulus-response curves from the currents shown in b. d Mean ± S.D. peak sensory transduction current amplitudes from apical FM1-43-negative and FM1-43-positive IHCs and OHCs from Tmc1^∆/∆;Tmc2^∆/∆ mice injected with sAAV-Tmc1 at time points as indicated. n = number of hair cells