Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2019 Jan 22;10:236. doi: 10.1038/s41467-018-08264-w

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 4 — sAAV-Tmc1 restores neuronal responses in auditory cortex of Tmc1^∆/∆ mice. a (Top) Schematic of multi-unit (MU) activity recordings in the auditory cortex. (Below) Representative raster plots from each group. Data were recorded from six C57B/L6 wild-type control mice and eleven awake head-fixed Tmc1^∆/∆ mice injected with sAAV-Tmc1. Tmc1^∆/∆-injected mice were divided into two groups, those with significant improvement in ABR thresholds (ABR+: thresholds ≤70 dB at any frequency; n = 7 mice) and mice with little improvement in thresholds (ABR−: thresholds ≥75 dB at all frequencies; n = 4 mice). Sound stimulus duration indicated as gray bar. BBN: broad band noise, ACtx: auditory cortex. b Quantification of spontaneous activity for WT control mice and Tmc1^∆/∆-injected mice (top). Quantification of both threshold (middle) and latency (bottom) resulting from broad band noise (BBN) stimuli presentation at different sound levels. c Representative frequency response areas after presentation of 361 frequency-level sound combinations. d Distribution of population tuning properties for responsive units Illustrating the best frequency (tip) and bandwidth measured 10 dB above threshold (side lines). Distribution of pure tone thresholds (bottom, right) and best frequencies (top, left) for responsive units. (Top, right) Bandwidth tuning responses for the stimulus presentation in (C). e (Top left) Schematic of recordings from auditory cortex during presentation of a visual stimulus consisting of gratings with changing spatial frequency. (Right) Preferred spatial frequency among ABR+, ABR−, and control mice. f Quantification of visually evoked onset latency in ABR− mice, ABR+ and control mice. g Representative raster plots from neurons recorded from the paradigm in f. h Startle response amplitudes normalized to mouse weight measured at P28-P30 and plotted as a function of sound intensity and as mean ± S.D. for 6 control C57BL/6 mice (black), ten individual Tmc1^∆/∆ mice injected with sAAV-Tmc1 (light green, mean ± S.D. in bold green), and five uninjected Tmc1^∆/∆ mice (red)