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. 2019 Jan 22;9:284. doi: 10.1038/s41598-018-36489-8

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Gene expression of tight junction proteins in whole brains from IAE model mice. (a) The gene expressions of 84 cell junction proteins in whole brains from the control and IAE (Flu + LPS) groups at 8 h post LPS administration were analysed by PCR array. The fold regulation threshold was 2. (b–e) The expression levels of Cav1 (b), Gjb1 (c), Gjc2 (d), and Cdh1 (e) in whole brains at 8 h and 24 h post LPS administration were analysed. Data are shown as the mean ± SEM (8 h control, n = 5; Flu, n = 3; LPS, n = 5; Flu + LPS, n = 5; 24 h control, n = 5; Flu, n = 5; LPS, n = 3; Flu + LPS, n = 6) and are from a representative experiment of two or three independent experiments. NS; not significant. *p < 0.05; **p < 0.01; ***p < 0.001.