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. 2019 Jan 22;9:307. doi: 10.1038/s41598-018-36425-w

Figure 2.

Figure 2

Overexpression of miR-181a in Scp-1 cells increases adipogenesis. (A) Oil red O staining for Scp-1 cells overexpressing either scramble vector (p000) or miR-181a (p181a) on day 14 of adipogenic differentiation with representative micrographs (top) and quantification of Oil red O staining for Scp-1 p000 and p181a cells at day 14 (bottom). (B) Graph depicting increased expression of miR-181a at days 0 and 7 in Scp-1 p181a vs p000 cells. (C) Graphs depicting increased mRNA expression of PPARG and CEBPA at day 0 and increased expression of adipogenesis target genes at day 7 in Scp-1 p181a vs p000 cells. (D) Representative western blot images showing increased expression of CEBPA and PPARG at day 0 in Scp-1 p181a vs p000 cells with quantification (right) (Full blots are shown in Supplemental Fig. 6A). (E) Representative western blot images showing increased expression of CEBPA, PPARG, and FABP4 at day 7 in Scp-1 p181a vs p000 cells with quantification (right) (Full blots are shown in Supplemental Fig. 6B). Data are representative of 3 independent experiments. * is p ≤ 0.05, ** is p ≤ 0.005, *** is p ≤ 0.0005.