Fig. 5.

Increased bypass of hairpin DNA structures by Polε-P301R. The polymerase and DNA substrate concentrations are as in Fig. 3a. a DNA polymerase activity was assayed on P50/T80H oligonucleotide substrate containing 6-bp inverted repeats in the template region, and the fraction of products longer than 71 nt indicating hairpin bypass was quantified. Representative of three independent experiments. b DNA polymerase activity was assayed on P51T/T80H oligonucleotide substrate containing inverted repeats in the template region and a terminal G-T mismatch, and the fraction of products longer than 71 nt indicating hairpin bypass was quantified. Representative of four independent experiments. c Polε-P301R was incubated with the P51T/T80H substrate for 30 min, and relative efficiency of mismatch extension vs. proofreading was determined by BsaJI digestion of the reaction products as in Fig. 4b. One experiment. Source data are provided in a Source Data file