TABLE 1.
Primers used in this study
| Primer name | Primer sequence | Purpose | PCR product size (bp) |
|---|---|---|---|
| 0195-706/707s-IBS | AAAAAAGCTTATAATTATCCTTAGGATTCGTGAAGGTGCGCCCAGATAGGGTG | pJIR750_0195i construction | 350 |
| 0195-706/707s-EBS1d | CAGATTGTACAAATGTGGTGATAACAGATAAGTCGTGAAGTATAACTTACCTTT CTTTGT |
pJIR750_0195i construction | |
| 0195-706/707s-EBS2 | TGAACGCAAGTTTCTAATTTCGATTAATCCTCGATAGAGGAAAGTGTCT | pJIR750_0195i construction | |
| 0195KOF | CATAAAACAATTTCATCAGTAGCAG | Screen for intron insertion in cpr_0195 |
350 |
| 0195KOR | AAAACTCCACTTATAGCCAATAAAA | Screen for intron insertion in cpr_0195 |
|
| 1055-433/434s-IBS | AAAAAAGCTTATAATTATCCTTAAATAACG-ATATAGTGCGCCCAGATAGGGTG | pJIR750_1055i construction | 350 |
| 1055-433/434s-EBS1d | CAGATTGTACAAATGTGGTGATAACAGATAAGTCGATATATTTAACTTACCTTT CTTTGT |
pJIR750_1055i construction | |
| 1055-433/434s-EBS2 | TGAACGCAAGTTTCTAATTTCGGTTTTATTCCGATAGAGGAAAGTGTCT | pJIR750_1055i construction | |
| 1055Fscreen | CTTTTAATCATATTGACAGAAAA | Screen for intron insertion in cpr_1055 |
319 |
| 1055Rscreen | TTAGGATGGATTCTATCTAATTTTAC | Screen for intron insertion in cpr_1055 |
|
| 0195compF | CATCATGGATCCGATACATTTAATAATTCTGCAAATTCTTTAGAC | pJIR750_0195comp construction | 3,474 |
| 0195compR | CATCATGGATCCGAGACAAAAGTAAGTTTTGATATACC | pJIR750_0195comp construction | |
| PJIR750Screen | CTGCAGGTCGACTCTAGA | Screen for pJIR750_ 0195comp in Strain 13 |
|
| 0195RT-F | CAAAGAAATCTTTAGATGATTGCGA | RT-PCR analysis of cpr_0195 | 329 |
| 0195RT-R | GATAGAAAACTTGGAGTTCATTTTGTAG | RT-PCR analysis of cpr_0195 | |
| 1055RT-F | GCAAAGGTTTAAATGAGAGTTTTAG | RT-PCR analysis of cpr_1055 | 282 |
| 1055RT-R | TATAGAATATATTCTCTGCTATTCCAC | RT-PCR analysis of cpr_1055 | |
| PolCF | ACTTCCCTGCAAGCCTCTTCTCCT | RT-PCR analysis of polC | 393 |
| PolCR | TGGTTCAGCTTGTGAAGCAGGGC | RT-PCR analysis of polC | |
| rSpo0AHisF | CCCCTCTAGAAATAATTTTGTTTAACTTTAAGAAGGAGATATACCATGAAGGAAT CAAAAATATCTGTAT |
Cloning of spo0A gene for recombinant expression |
828 |
| rSpo0AHisR | GTGCTCGAGGCTAACTTTATTCTTTAGTCTTAATTTATC | Cloning of spo0A gene for recombinant expression |
|
| r0195HisF | CCCCTCTAGAAATAATTTTGTTTAACTTTAAGAAGGAGATATACCATGGAAATTA CGACTAAAAACAAAAAAAG |
Cloning of cpr_0195 gene for recombinant expression |
1,543 |
| r0195HisR | GTGCTCGAGATGTACTTCATAAATATCAGAAAGCTC | Cloning of cpr_0195 gene for recombinant expression |
|