Fig. 7.
Survival of Lactobacillus plantarum group strains under acidic conditions.
L. plantarum strains AWA1401, IYO1401, and JCM1149T precultured in MRS broth (pH 6.2) were added at 10% (v/v) into MRS broth with a pH of 2 or 3. The preculture suspensions were also added to normal MRS broth (pH 6.2) at 10%. Each mixture was incubated for 2 hr at 37°C, and then serially diluted cultures were spread on MRS plates. After anaerobic incubation overnight at 37°C, colony-forming units (CFUs) were estimated by counting. The survival rate (calculated from CFUs) in a pH 6.2 medium was set as 100%. **p<0.01 (compared with pH 6.2; Dunnett’s test, ANOVA).