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. 2018 Dec 12;8(1):99–108. doi: 10.1021/acssynbio.8b00342

Figure 6.

Figure 6

Secretion of IsaA by B. subtilis 168 or PG10 in the presence of protease inhibitors. Production of IsaA in B. subtilis 168 and PG10 was assessed as described for Figure 5, but in this case, the growth medium was supplemented with the Complete protease inhibitor without EDTA cocktail from Roche. Production of IsaA was induced with 1% subtilin (t = 0), and samples were collected as a function of the time indicated. The cytoplasmic protein TrxA was used as a marker for cell lysis. The positions of TrxA and the precursor and mature forms of IsaA are indicated with arrow heads.