Table 5.
LV protein levels (immunoblotting)
| p | |||||
|---|---|---|---|---|---|
| Protein | Con (4) | HF (4) | HF + UCn3 (4) | Control vs. HF | HF vs. HF + UCn3 |
| p-PKA-RIIa | 0.6 ± 0.1 | 0.7 ± 0.1 | 0.6 ± 0.1 | 1 | 0.6 |
| p-PKA-RIIb | 0.3 ± 0.15 | 0.3 ± 0.03 | 0.2 ± 0.03 | 1 | 0.8 |
| Total-PKA | 0.4 ± 0.01 | 0.3 ± 0.02 | 0.3 ± 0.02 | 1 | 0.8 |
| p-PKA/Total | 1.8 ± 0.3 | 2.0 ± 0.10 | 1.9 ± 0.1 | 1 | 0.8 |
| CamKII | 0.6 ± 0.1 | 0.5 ± 0.1 | 0.6 ± 0.1 | 1 | 0.8 |
| p-CamKII | 0.5 ± 0.1 | 0.4 ± 0.02 | 0.6 ± 0.1 | 0.8 | 0.2 |
| p-CamKII/Total | 0.9 ± 0.15 | 0.9 ± 0.1 | 1.1 ± 0.2 | 1 | 1 |
| p-RYR2/Total | 2.2 ± 0.4 | 0.7 ± 0.1 | 1.2 ± 0.1 | 0.01 | 0.06 |
| p16-PLB | 0.1 ± 0.1 | 0.7 ± 0.1 | 0.9 ± 0.11 | 1 | 0.8 |
| p16-PLB/Total | 3.7 ± 0.6 | 1.1 ± 0.2 | 2.1 ± 0.5 | 0.01 | 0.2 |
| p17-PLB | 6.3 ± 0.7 | 2.4 ± 0.8 | 2.2 ± 0.4 | 0.02 | 1 |
| p17-PLB/Total | 28.6 ± 3.5 | 3.5 ± 1.1 | 5.4 ± 1.5 | 0.001 | 0.6 |
| CARP | 10.3 ± 1 | 14 ± 1.8 | 14 ± 1.1 | 0.2 | 1 |
| MYLK3 | 0.6 ± 0.1 | 1.0 ± 0.3 | 0.7 ± 0.1 | 0.4 | 0.8 |
Immunoblotting data presenting protein levels normalized for GAPDH or vinculin. Data are mean ± SE. p-Values from a Student's t-test (unpaired, two-tailed) with Bonferroni correction for multiple comparisons; numbers in parentheses indicate group size.
p-TnI, S23/24 phosphorylated troponin I; PKA-RIIa, protein kinase A IIa; CamKII, Ca2+/calmodulin-dependent protein kinase II; RYR, ryanodine receptor; PLB, phospholamban; MYLK3, myosin light chain kinase 3.