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. 2017 Jul 3;25(2):214–221. doi: 10.1177/1933719117716775

Figure 1.

Figure 1.

Regulation of PR-A/B-mediated progesterone responsiveness in human myometrial cells by serum factors. A, Effect of FBS (5.0% or 0.5%) on progesterone (P4)-induced PRE-LUC activity in hTERT-HMA/B cells conditioned to express PR-A (with DOX) and/or PR-B (with DAH). Cell were transiently transfected with PRE-LUC expression DNA and condition to express PR-A, PR-B, or both overnight and then exposed to progesterone for 24 hours in various FBS media levels. Fetal bovine serum increased the capacity for PR-A to transrepress the activity of PR-B. B, Effect of FBS on progesterone (P4)-induced expression of FKBP5 in hTERT-HMA/B cells. Cells were conditioned to express PR-A and PR-B overnight in media containing 5% FBS. The following morning, the cells were washed and cultured in 5% FBS or serum-free (0% FBS) media containing progesterone (P4; 100 nM) or vehicle (ethanol) for 6 hours. The capacity for progesterone to increase FKBP5 mRNA abundance in cells expressing PR-A and PR-B was significantly decreased by FBS. All data are mean ± standard error of the mean (SE) (n = 3). FBS, fetal bovine serum; FKBP5, FK506 binding protein; mRNA, messenger RNA; PRE-LUC, progesterone response element–luciferase.