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Low oxygen increases 5T4 messenger RNA (mRNA) expression. A. Western blot analysis of 3 different samples of purified trophoblast cells cultured under ambient (control) or low-oxygen (2% O2) conditions. CTB (number) represents the deidentification code used to denote individual samples. B and C, Semiquantitative analysis of the effect of low oxygen on 5T4 expression on protein (B) and mRNA (C) expression; CTB indicates cytotrophoblast.
