Figure 5.

Ulipristal acetate (UPA) and mifepristone differentially regulate heart- and neural crest derivatives-expressed protein 2 (HAND2) messenger RNA (mRNA) expression in human endometrial stromal cells. Primary cultures of human stromal cells were grown in Dulbecco’s modified Eagle’s medium/F-12 medium containing 5% charcoal-stripped fetal bovine serum. The cells were treated with a hormone mixture containing 10 nM 17β-estradiol (E), 1 μM progesterone (P), 0.5 mM 8-bromo-cAMP, and 10 μM UPA, mifepristone, or vehicle for 6 days. Cells were harvested 2 days (left panel) or 6 days (right panel) after addition of hormone mixture. Total RNA was isolated and subjected to quantitative polymerase chain reaction (qPCR) using primers for HAND2. The level of Rplp0 was used as an internal control to normalize gene expression. The values are presented as the mean fold induction ± SEM, P < .05.