Figure 7.

Ulipristal acetate (UPA) and mifepristone differentially regulate HAND2 messenger RNA (mRNA) expression in human endometrial stromal cells. Primary cultures of human stromal cells were treated with a hormone mixture containing 10 nM 17β-estradiol (E), 1 μM progesterone (P), 0.5 mM 8-bromo-cAMP, and 5 μM UPA, mifepristone, or vehicle for 6 days. Cells were harvested 2, 3, 4, 5, and 6 days after addition of hormone mixture. Total RNA was isolated and subjected to quantitative polymerase chain reaction (qPCR) using primers for HAND2. The level of Rplp0 was used as an internal control to normalize gene expression. The values are presented as the mean fold induction ± SEM, *P < .05, **P < .01.