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. 2019 Jan 22;7:17. doi: 10.1186/s40425-019-0507-2

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© The Author(s). 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 1 — The subtypes of ex vivo expanded DNTs. a Fold expansion of DNTs on day 14 was calculated. b Numbers of DNTs expanded from 1 ml blood were calculated. c, d The purity of DNTs was detected on day 0 and day 14. e The percentages of αβ- and γδ-subtypes of DNTs were detected by flow cytometry. The subtypes of γδ-DNTs and αβ-DNTs were detected using Valpha24 (iNKT cell TCR) and Valpha7.2 (MAIT TCR)