Fig. 4.

Cytotoxicity of DNTs against NSCLC cells can be mediated by different mechanisms. a The percentage of reduced target cell lysis by a specific antibody was compared to those in the presence of the corresponding isotype control antibody. b The percentage of reduced target cell lysis by CMA treatment with or without anti-TRAIL antibody was compared to those in the presence of vehicle DMSO and isotype controls. c NSCLC cell lines were cultured in media or IL-2 conditioned DNT culture supernatant in the presence of anti-TRAIL neutralizing antibody or isotype control. The death of NSCLC cells was determined by flow cytometry. c Different concentrations of rhsTRAIL were added to NSCLC cell cultures. The death of lung cancer cells was determined by flow cytometry. Statistical differences were calculated using one-way ANOVA followed by Bonferroni’s post hoc test. ns, not significant, *P < 0.05, **P < 0.01, ***P < 0.001. Data from one of three representative experiments (a) or cumulative of six independent experiments (b) are shown