Table 1.
NSCLC cell lines have different susceptibilities towards ex vivo expanded DNTs
| Cell line (Cell source) | % cytotoxicity (Mean ± SD) at different E:T ratios | E:T EC50 | |||
|---|---|---|---|---|---|
| 20:01 | 10:01 | 5:01 | 2.5:01 | ||
| H2279 (ATCC) | 97.22 ± 12.04 | 87.69 ± 11.65 | 47.69 ± 19.34 | 23.83 ± 13.53 | 4.03 |
| H460 (ATCC) | 89.27 ± 7.23 | 61.52 ± 8.30 | 30.09 ± 12.88 | 14.88 ± 8.75 | 4.86 |
| 12 (PDX) | 83.43 ± 17.97 | 75.32 ± 18.10 | 50.66 ± 21.09 | 24.15 ± 10.97 | 5.76 |
| 178 (PDX) | 83.03 ± 10.55 | 58.96 ± 12.30 | 34.43 ± 7.49 | 11.81 ± 2.83 | 6.7 |
| 426 (PDX) | 83.00 ± 10.50 | 64.54 ± 13.74 | 40.79 ± 7.24 | 16.32 ± 4.46 | 7.47 |
| 277 (PDX) | 81.25 ± 9.27 | 67.62 ± 14.95 | 47.25 ± 8.07 | 21.76 ± 6.73 | 7.56 |
| 655 (PDX) | 81.14 ± 7.99 | 63.21 ± 8.85 | 31.84 ± 1.90 | 17.79 ± 1.77 | 7.97 |
| 229 (PDX) | 80.64 ± 10.41 | 58.96 ± 10.73 | 27.98 ± 14.16 | 10.28 ± 5.43 | 8.86 |
| H125 (ATCC) | 78.62 ± 14.80 | 53.97 ± 19.28 | 24.05 ± 10.39 | 16.39 ± 8.94 | 9.53 |
| 239 (PDX) | 63.57 ± 9.98 | 36.19 ± 16.83 | 11.82 ± 8.76 | 12.45 ± 3.81 | 16.8 |
| A549 (ATCC) | 60.12 ± 10.51 | 35.69 ± 5.41 | 18.28 ± 5.21 | 9.77 ± 4.50 | 16.93 |
| 137 (PDX) | 53.66 ± 15.40 | 30.84 ± 13.30 | 16.67 ± 6.47 | 8.51 ± 2.90 | 20.57 |
Primary and established human NSCLC cell lines were cocultured with ex vivo expanded DNTs at various E:T ratios. The percentages of specific cytotoxicity against target cells were detected. E:T EC50 was calculated for each cell line