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. 2019 Jan 22;17:13. doi: 10.1186/s12958-019-0457-z

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© The Author(s). 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 2 — Effects of HFD during gestation in early follicular development. Representative pictures of neonatal ovaries from offspring rats from control (a) and HFD mothers (b) (400X). Kisspeptin-IR was detected mainly in the cytoplasm of oocytes (c and d) and FSHR-IR located in granulosa cells (e and f). The count results of various follicles at PND4 were shown in g and the MOD for kisspeptin and FSHR was summarized in h. values are the means ± S.E.M (n = 3–6 for each group). *P < 0.05 HFD vs NCD