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. 2019 Jan 22;10:40. doi: 10.1186/s13287-019-1139-7

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© The Author(s). 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 3 — Differentially expressed genes in RNA-Seq were confirmed by Q-PCR. A total of 20 genes, including 10 SCOS Sertoli cells downregulated (FGF5, DKK1, IGF2, PNP, SEMA3A, CDH1, SPRY2, CDK6, MET and WNK4) (a) and upregulated (b) ones (SPP1, TP53I11, ADRA2A, PLA2G2A, NTN1, OSCAR, PRRX2, WFDC1, NGFR and MT3), from 308 DEGs were selected to validate the results of RNA-Seq. Note: OA obstructive azoospermia, SCOS Sertoli cell-only syndrome, SC Sertoli cell. Asterisk indicated statistically significant differences (P < 0.05)